RESEARCH ASSISTANT III- Jardine Lab

ABOUT US: Scripps Research is ranked one of the most influential institutions in the world for its impact on innovation. We expand basic knowledge in the biosciences and use these fundamental advancements to develop profound innovations that improve wellbeing. Our educational and training programs mold talented and committed students and postdocs into leading edge scientists. Scientists in the institute’s six academic research departments work hand-in-hand with researchers of the Scripps Research Translational Institute and Calibr to merge foundational studies in biology, chemistry and computer science with translational science to produce pioneering drugs and advances in digital and precision medicine. Together, we cultivate new scientific leaders and expand the frontiers of knowledge to deliver medical breakthroughs to better human health around the globe. If you have a passion for making a difference, this could be your opportunity to join our transformative team.

POSITION TITLE:  Research Assistant III

POSITION SUMMARY:

The Jardine lab is seeking a Research Associate III to join us in the Department of Immunology and Microbiology at Scripps Research. We utilize engineering-based approaches to understand how the adaptive immune system recognizes diverse antigens and apply these insights to design antibodies and vaccines for challenging targets. We are actively seeking a highly motivated candidate to contribute to our discovery efforts.

Primary responsibilities of this position will be the production, screening, and sequencing of yeast display libraries. This role will be part of a larger collaborative team, but the ideal candidate should be able to plan, conduct and troubleshoot experiments largely independently. Given the interdisciplinary nature of the work, particularly exceptional candidates will have the opportunity to broaden their responsibilities. This may include expanding into additional areas like informatics analysis of deep-sequenced libraries or downstream characterization of selected molecules.

RESPONSIBILITIES AND DUTIES:

• Recombinant expression of proteins and antibodies baits and probes:
  • DNA preparation
  • Transient transfections in Expi293
  • Standard affinity (Nickel, protein A, etc) and SEC purifications
  • Fluorophore conjugations and QC of produced material
• Production and screening of yeast display libraries
  • PCR library preparation
  • Yeast transformations
  • FACS screening of positive clones
  • Harvesting of DNA from sorted populations
• Deep sequencing preparation
  • DNA amplicon preparation
  • QC of DNA prior to sequencing
  • Assist in the design of novel assays.
  • Deliver protein preparations and data analysis packages to collaborating scientists on deadline
  • Manage and maintain documentation for all produced materials
  • Organize effectively and discuss data
  • Help troubleshoot any issues.

REQUIREMENTS:

• Requires a bachelor’s degree in a relevant scientific discipline or an equivalent combination of education, training and/or experience from which comparable knowledge, skill and abilities have been attained.
• Requires a minimum of 5 years’ experience.
• Candidates possessing a master’s degree, 3+ years’ experience required.

PHYSICAL REQUIREMENTS:

This position works in a laboratory environment. Further details of the physical requirements of established essential functions for this position will be addressed/discussed during the interview process.

COMPENSATION:  The expected hiring range for this position is $68,000 - $81,600 per year, commensurate with experience. Consideration will be given to experience that exceeds the listed requirements.

COMPREHENSIVE BENEFITS INCLUDE:
• Employer Contributed Retirement Plan – Depending on eligibility, employees receive an employer contributed retirement plan (no employee contribution required) and the option to contribute to a 403(b) (which is similar to a 401(k) using your own pre or post-tax dollars)
• Full Suite of Health and Welfare plans including three medical plan options (including an HSA available option), dental, vision, life insurance, disability, EAP and more
• Access to Flexible Spending Accounts (Medical/Dependent Care)
• Competitive vacation and sick leave policies
• Free, on-site parking

The above statements describe the level of work performed and expected in general terms. The statements are not intended to list all the responsibilities, duties and/or skills required of employees so classified, and the content herein is subject to change due to the business needs of Scripps Research, with or without notice. Furthermore, nothing in this job description shall be interpreted to be in conflict with or to eliminate or modify in any way the employment-at-will status of Scripps Research staff.

EEO Statement:
The Scripps Research Institute is an Equal Opportunity Employer. We promote diversity of thought, culture, and background in the fields of science. All qualified applicants are encouraged to apply and will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, protected veteran status, or any other legally protected characteristic or status. Underrepresented individuals are encouraged to apply.